Immunoaffinity Purification of Dietary Heterocyclic Amine Carcinogens
نویسندگان
چکیده
منابع مشابه
Immunoaffinity purification of dietary heterocyclic amine carcinogens.
Cooking meats produces a family of heterocylic aromatic amines that are carcinogens in rodents and genotoxic in many short-term assays. Concern that these compounds may be human carcinogens has prompted us to develop immunochemical methods for quantifying these compounds in the human diet and for identifying the parent compounds and metabolites in urine and feces. Previously reported monoclonal...
متن کاملRethinking the role of intestinal microflora in bioactivation of food-borne heterocyclic amine carcinogens.
Since the discovery that mutagenic and carcinogenic heterocyclic amines are formed during the cooking of foods (7,2), it has been widely hypothesized that intestinal microflora are likely to play an important role in the bioactivation of these amines (3), leading to tumor induction in the colon of humans and experimental animals. In this issue of the Journal, Weisburger et al. (4) provide stron...
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To investigate a method to reduce the amount of mutagenic/carcinogenic heterocyclic aromatic amines formed during frying of ground beef, we measured both the mutagenic activity in Salmonella strain TA98 and the amount of known heterocyclic amines by solid-phase extraction and HPLC. The beef patties received microwave treatment for various times before frying. Microwave pretreatment for 0, 1, 1....
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The bioavailability and the bioreactivity of the carcinogenic heterocyclic amine [2-14C]2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine (PhIP) have been investigated at a dose approximating that likely from the human diet by accelerator mass spectrometry (AMS). [2-14C]PhIP was administered to mice at a dose equivalent ot the consumption of two 100 g beef patties (41 ng/kg). The biological half...
متن کاملPhytochrome immunoaffinity purification.
We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, is...
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ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1993
ISSN: 0091-6765
DOI: 10.2307/3431502